Metagenome of cold geyser Wallender Born - SRA

SRX21221950: Metagenome of cold geyser Wallender Born
1 ILLUMINA (NextSeq 500) run: 105M spots, 31.5G bases, 12.2Gb downloads

Design: DNA extraction from filter samples via Powersoil Max kit of qiagen, library preparation via Westburg NGS DNA Library Prep Kit (cat. no. WB 9096) without PCR amplification. Insert size validation to be more than 400 bp. Sequencing with 2x 150bp paired-end reads on a NovaSwq500 platform to an approximate sequencing depth of 20 Gbp.

Submitted by: University Duisburg-Essen

Study: Genome-resolved metagenomics of the cold-water geyser Wallender Born

PRJNA1001268 • SRP452921 • All experiments • All runs

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In this project, a 12-day time series of the cold-water geyser Wallender Born in the Volcanic Eiffel (Germany) was sampled. The recovered water was filtered sequentially on 0.2um and 0.1um pore size filters. Additionally, a bulk 0.1um pore size filter sample was taken to recover the entire community in a single fraction. The DNA extracted from the filter samples was to illumina sequencing, followed by genome-resolved metagenomics.

Sample: groundwater was collected from the erupting geyser and filtered sequentially on 0.2um and then 0.1 um pore size filters. Geochemical parameters were determined in duplicate and values depicted here are averages. 0.1µm and 0.2µm samples have same biochemical parameters. Some samples were taken as bulk filters, i.e., direct filtering on 0.1µm pore size filters without previous 0.2 µm pore size filtering to capture the entire community.

SAMN36797821 • SRS18478884 • All experiments • All runs

Organism: groundwater metagenome

Library:

Name: 25

Instrument: NextSeq 500

Strategy: WGS

Source: METAGENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 105M spots, 31.5G bases, 12.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR25490534	105,015,567	31.5G	12.2Gb	2023-12-15

ID:: 28658446

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